Abstract
Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.
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References
Saito T, Nakatsuji N (2001) Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240(1):237–246
Matsuda T, Cepko CL (2007) Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci U S A 104(3): 1027–1032
Barnabe-Heider F et al (2008) Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nat Methods 5(2): 189–196
Konishi Y, Stegmuller J, Matsuda T, Bonni S, Bonni A (2004) Cdh1-APC controls axonal growth and patterning in the mammalian brain. Science 303(5660):1026–1030
Saijilafu, Zhang BY, Zhou FQ (2013) Signaling pathways that regulate axon regeneration. Neurosci Bull 29(4):411–420
Saijilafu, Hur EM, Zhou FQ (2011) Genetic dissection of axon regeneration via in vivo electroporation of adult mouse sensory neurons. Nat Commun 2:543
Hur EM et al (2011) GSK3 controls axon growth via CLASP-mediated regulation of growth cone microtubules. Genes Dev 25(18): 1968–1981
Liu CM et al (2013) MicroRNA-138 and SIRT1 form a mutual negative feedback loop to regulate mammalian axon regeneration. Genes Dev 27(13):1473–1483
Acknowledgments
This work was supported by grants to F.Q.Z. from NIH (R01NS064288) and the Craig H. Neilsen Foundation.
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Saijilafu, Zhang, BY., Zhou, FQ. (2014). In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration. In: Murray, A. (eds) Axon Growth and Regeneration. Methods in Molecular Biology, vol 1162. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0777-9_14
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DOI: https://doi.org/10.1007/978-1-4939-0777-9_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0776-2
Online ISBN: 978-1-4939-0777-9
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