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Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs

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Animal Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1161))

Abstract

Influenza virus isolation is a procedure to obtain a live and infectious virus that can be used for antigenic characterization, pathogenesis investigation, and vaccine production. Embryonated chicken egg inoculation is traditionally considered the “gold standard” method for influenza virus isolation and propagation. However, many primary cells and continuous cell lines have also been examined or developed for influenza virus isolation and replication. Specifically, swine influenza virus (SIV) isolation and propagation have been attempted and compared in embryonated chicken eggs, some primary porcine cells, and a number of continuous cell lines. Currently Madin–Darby canine kidney (MDCK) cells remain the most commonly used cell line for isolation, propagation, and titration of SIV. Virus isolation in embryonated chicken eggs or in different cell lines offers alternative approaches when SIV isolation in MDCK cells is unsuccessful. Nasal swabs, lung tissues, and oral fluids are three major specimen types for SIV isolation. In this chapter, we describe the procedures of sample processing, SIV isolation in MDCK cells and in embryonated chicken eggs, as well as methods used for confirming the virus isolation results.

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Author information

Authors and Affiliations

  1. Veterinary Diagnostic Laboratory, Iowa State University, 1600 South 16th Street, Ames, IA, 50011, USA

    Jianqiang Zhang & Phillip C. Gauger

Authors
  1. Jianqiang Zhang
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  2. Phillip C. Gauger
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Correspondence to Jianqiang Zhang .

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Editors and Affiliations

  1. Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, Georgia, USA

    Erica Spackman

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Zhang, J., Gauger, P.C. (2014). Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 1161. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0758-8_22

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  • DOI: https://doi.org/10.1007/978-1-4939-0758-8_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0757-1

  • Online ISBN: 978-1-4939-0758-8

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