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Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus

Part of the Methods in Molecular Biology book series (MIMB,volume 1158)


Using a perennial model plant allows the study of reoccurring seasonal events in a way that is not possible using a fast-growing annual such as Arabidopsis thaliana (Arabidopsis). In this study, we present a hybrid aspen (Populus tremula × P. tremuloides) as our perennial model plant. These plants can be grown in growth chambers to shorten growth periods and manipulate day length and temperature in ways that would be impossible under natural conditions. In addition, the use of growth chambers allows easy monitoring of height and diameter expansion, accelerating the collection of data from new strategies that allow evaluation of promoters or inhibitors of growth. Here, we describe how to study and quantify responses to seasonal changes (mainly using P. tremula × P. tremuloides) by measuring growth rate and key events under different photoperiodic cycles.

Key words

  • Photoperiod
  • Populus
  • Perennial
  • Critical day length
  • Bud set
  • Bud burst
  • Freezing tolerance
  • Cold acclimation

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  • DOI: 10.1007/978-1-4939-0700-7_21
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The authors are grateful for financial and other support from various funding bodies and institutions. M.J. is supported by a scholarship from the Alexander von Humboldt Foundation and through SPP1530 (DFG). C.I. is supported by FONDECYT grant no. 1110831 (CONICYT—Chile).

M.E.E. is a VINNMER Marie Curie International Qualification Fellow funded by the Swedish Governmental Agency for Innovation Systems (VINNOVA) and the European Union, with current support from the Swedish Research Council (VR), Umeå University Career Grant, and Carl Trygger’s Foundation for Scientific Research. Further M.E.E. would like to acknowledge the Department of Plant Sciences and Churchill College at Cambridge University, Umeå Plant Science Centre, and support from the VR- and VINNOVA-funded Berzelii Centre of Forest Biotechnology, FORMAS, the Kempe Foundation, and Nils och Dorti Troëdssons forskningsfond.

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Correspondence to Mikael Johansson .

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Johansson, M., Takata, N., Ibáñez, C., Eriksson, M.E. (2014). Monitoring Seasonal Bud Set, Bud Burst, and Cold Hardiness in Populus . In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY.

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