Abstract
Automated liquid chromatography tandem mass spectrometry (LC-MS/MS) is a well-established technique for identification of components from complex mixtures in shotgun proteomics experiments. Approaches involving the use of matrix-assisted laser desorption/ionization mass spectrometry (LC-MALDI-MS/MS) comprise the preparation of protein extracts, their enzymatic digestion, the separation of the resulting peptides by nanoscale liquid chromatography coupled to a collector that deposits the microfractions onto a MALDI plate, and finally the mass spectrometry analysis of the fractions. Using an LC-MALDI strategy, the rate of the collection of MS/MS data is decoupled from the chromatographic separation, thus allowing high-quality data which are often complementary to electrospray ionization (LC-ESI-MS/MS) techniques.
Key words
- Mass spectrometry
- MALDI
- Liquid chromatography
- Proteomics
- Proteins
- Peptides
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Acknowledgement
Our work is supported by grants from Fondo Investigación Sanitaria-Spain (CP09/00114, PI11/02397, PI12/00329) and Secretaría I + D + I Xunta de Galicia (10CSA916058PR). J. Mateos (CA11/00050) and P. Fernández-Puente (CA09/00458) are supported by Fondo Investigación Sanitaria-Spain. C. Ruiz-Romero is supported by the Miguel Servet program from Fondo Investigación Sanitaria-Spain (CP09/00114).
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Fernández-Puente, P., Mateos, J., Blanco, F.J., Ruiz-Romero, C. (2014). LC-MALDI-TOF/TOF for Shotgun Proteomics. In: Martins-de-Souza, D. (eds) Shotgun Proteomics. Methods in Molecular Biology, vol 1156. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0685-7_2
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DOI: https://doi.org/10.1007/978-1-4939-0685-7_2
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