One of the TNF Superfamily Members: Bifunctional Protein, TNFR2-Fc-IL-1ra
Many anti-inflammatory agents have been exploited for the treatment of inflammatory diseases by targeting the most potent proinflammatory cytokines including tumor necrosis factor (TNF) and interleukin-1 (IL-1). Theoretically, simultaneous neutralization or blocking two important inflammatory mediators might achieve a synergistic therapeutic effect. A recombinant fusion protein, TNFR2-Fc-IL-1ra (TFI), was developed as bifunctional inflammatory inhibitor. TFI was able to strongly neutralize TNF activity and to antagonize IL-1 receptor in the cell binding inhibition assays, suggesting that TFI could be used as a bifunctional ligand with enhanced anti-inflammatory effect.
Key wordsProinflammatory cytokines Bifunctional protein Synergistic therapeutic effect Bioreactor Affinity Expression and purification
This work was supported by 863 grant 2012AA02A406 and State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences.