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Human Fertility pp 261–268Cite as

General Principles of Cryopreservation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1154))

Abstract

Cryopreservation quickly became a cornerstone technology in assisted reproduction because the banking of gametes, embryos, and gonadal tissues has increased the effectiveness of assisted reproductive technology cycles for infertility treatment as well as fertility preservation for patients at risk of premature sterilization. Cryopreservation protocols, both slow/equilibrium cooling and vitrification methods, have evolved empirically and still depend heavily on operator skill, but further automation promises to improve reproducibility and uniformity of results.

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Author information

Authors and Affiliations

  1. Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA

    Roger G. Gosden Ph.D.

Authors
  1. Roger G. Gosden Ph.D.
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Corresponding author

Correspondence to Roger G. Gosden Ph.D. .

Editor information

Editors and Affiliations

  1. The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA

    Zev Rosenwaks

  2. Dept. Developmental & Regenerative Biology, Ichan School of Medicine at Mount Sinai, New York, New York, USA

    Paul M. Wassarman

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© 2014 Springer Science+Business Media New York

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Gosden, R.G. (2014). General Principles of Cryopreservation. In: Rosenwaks, Z., Wassarman, P. (eds) Human Fertility. Methods in Molecular Biology, vol 1154. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0659-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-0659-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0658-1

  • Online ISBN: 978-1-4939-0659-8

  • eBook Packages: Springer Protocols

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