Abstract
Polyisoprenoid alcohols are representatives of high-molecular terpenoids. Their hydrocarbon chains are built of 5 to more than 100 isoprene units giving rise to polymer molecules that differ in chain-length and/or geometrical configuration. Plants have been shown to accumulate diverse polyisoprenoid mixtures with tissue-specific composition. In this chapter, methods of analysis of polyisoprenoid alcohols in plant material are described, including isolation and purification of polyisoprenoids from plant tissue, fast semiquantitative analysis of the polyisoprenoid profile by thin-layer chromatography (straight phase adsorption and reversed phase partition techniques), and quantification of polyisoprenoids with the aid of high performance liquid chromatography. This approach results in full characterization of complex polyisoprenoid mixtures accumulated in various plant tissues and other matrixes.
Keywords
- Polyisoprenoids
- Polyprenol
- Dolichol
- TLC
- HPLC
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Acknowledgements
This investigation was supported by a grant funded by the Polish National Cohesion Strategy Innovative Economy [UDA-POIG 01.03.01-14-036/09] and the School of Molecular Biology IBB PAS to K.G. We would like to thank Agata Lipko, IBB PAS for her help with TLC analyses.
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Gawarecka, K., Swiezewska, E. (2014). Analysis of Plant Polyisoprenoids. In: Rodríguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_9
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DOI: https://doi.org/10.1007/978-1-4939-0606-2_9
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