Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS
Heterologous expression of plant metabolic enzymes in microorganisms is extensively used for identifying genes involved in their pathways and producing useful compounds. Here, we describe a plasmid-based yeast expression system that easily allows the expression of different triterpene biosynthetic genes in wild-type yeast, providing a useful platform for identifying their functions and facilitating combinatorial biosynthesis of triterpenoids.
Key wordsCombinatorial biosynthesis Cytochrome P450 monooxygenases (CYPs) Cytochrome P450 reductase (CPR) 2,3-Oxidosqualene Oxidosqualene cyclases (OSCs) Triterpenoid Triterpenol Yeast
This work was financially aid in part by Frontier Research Base for Global Young Researchers, Osaka University, supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT).
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