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Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS

  • Ery Odette Fukushima
  • Hikaru Seki
  • Toshiya Muranaka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1153)

Abstract

Heterologous expression of plant metabolic enzymes in microorganisms is extensively used for identifying genes involved in their pathways and producing useful compounds. Here, we describe a plasmid-based yeast expression system that easily allows the expression of different triterpene biosynthetic genes in wild-type yeast, providing a useful platform for identifying their functions and facilitating combinatorial biosynthesis of triterpenoids.

Key words

Combinatorial biosynthesis Cytochrome P450 monooxygenases (CYPs) Cytochrome P450 reductase (CPR) 2,3-Oxidosqualene Oxidosqualene cyclases (OSCs) Triterpenoid Triterpenol Yeast 

Notes

Acknowledgements

This work was financially aid in part by Frontier Research Base for Global Young Researchers, Osaka University, supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT).

References

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ery Odette Fukushima
    • 1
    • 2
  • Hikaru Seki
    • 2
  • Toshiya Muranaka
    • 2
  1. 1.Frontier Research Base for Global Young ResearchersGraduate School of Engineering, Osaka UniversityOsakaJapan
  2. 2.Department of BiotechnologyGraduate School of Engineering, Osaka UniversitySuita, OsakaJapan

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