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Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-arming Technology

  • Kouichi Kuroda
  • Mitsuyoshi UedaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1152)

Abstract

The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of proteins/peptides library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecular-weight polysaccharides. In addition, along with fluorescence-based activity evaluation, fluorescence-activated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated proteins/peptides library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display efficiency, and harvesting of the displayed proteins/peptides from cell surface.

Key words

Cell surface display Cell surface engineering Arming yeast Whole-cell biocatalyst Molecular tool for high-throughput screening Single cell analysis Yeast cell chip 

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  1. 1.Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Department of Applied BiochemistryGraduate School of Agriculture, Kyoto UniversitySakyo-kuJapan

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