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Tools for Genetic Engineering of the Yeast Hansenula polymorpha

  • Ruchi Saraya
  • Loknath Gidijala
  • Marten Veenhuis
  • Ida J. van der KleiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1152)

Abstract

Hansenula polymorpha is a methylotrophic yeast species that has favorable properties for heterologous protein production and metabolic engineering. It provides an attractive expression platform with the capability to secrete high levels of commercially important proteins. Over the past few years many efforts have led to advances in the development of this microbial host including the generation of expression vectors containing strong constitutive or inducible promoters and a large array of dominant and auxotrophic markers. Moreover, highly efficient transformation procedures used to generate genetically stable strains are now available. Here, we describe these tools as well as the methods for genetic engineering of H. polymorpha.

Key words

Genetic engineering Hansenula polymorpha Methylotrophic yeast Metabolic engineering Heterologous protein production 

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Ruchi Saraya
    • 1
  • Loknath Gidijala
    • 1
  • Marten Veenhuis
    • 1
  • Ida J. van der Klei
    • 1
    Email author
  1. 1.Molecular Cell Biology, Kluyver Centre for Genomics of Industrial FermentationGroningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningenThe Netherlands

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