Evolutionary Engineering of Yeast
Evolutionary engineering is an inverse metabolic engineering strategy which is based on increasing genetic diversity and screening large populations for desired phenotypes. This strategy is highly advantageous in certain situations over rational metabolic engineering approaches, since there is little or no requirement of detailed genetic background information for the trait of interest. Here, we describe the experimental methodology for selecting stress-resistant yeast strains via evolutionary engineering approach by either serial batch or chemostat cultivations.
Key wordsBatch selection Chemostat selection Evolutionary engineering Inverse metabolic engineering
We thank TÜBİTAK (project no: 109 T638, 105 T314), COST (Action no: CM0902), and ITU Research Funds (project no: 33237, 34200) for financial support of our evolutionary engineering research.
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