Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species
Synthetic communities can be used as model systems for the molecular examination of species interactions. Manipulating synthetic communities to create novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-products of another.
Key wordsSynthetic ecology Consortia Cooperation Cross-feeding Evolution
- 9.Vartoukian SR, Palmer RM, Wade WG (2010) Strategies for culture of “unculturable” bacteria. FEMS Microbiol Lett 309:1–7Google Scholar
- 13.Harcombe W (2010) Novel cooperation experimentally evolved between species. Evolution 64:2166–2172Google Scholar
- 16.Ishii K, Shiio I (1968) Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5′-phosphate dehydrogenase, xanthosine-5′-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis. J Biochem 63:661–669Google Scholar
- 17.Nierlich DP, Magasanik B (1965) Regulation of purine ribonucleotide synthesis by end product inhibition. The effect of adenine and guanine ribonucleotides on the 5′-phosphoribosyl-pyrophosphate amidotransferase of Aerobacter aerogenes. J Biol Chem 240:358–365Google Scholar
- 18.Lawrence DA, Smith DA, Rowbury RJ (1968) Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics 58:473–492Google Scholar