Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species

Part of the Methods in Molecular Biology book series (MIMB, volume 1151)

Abstract

Synthetic communities can be used as model systems for the molecular examination of species interactions. Manipulating synthetic communities to create novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-products of another.

Key words

Synthetic ecology Consortia Cooperation Cross-feeding Evolution 

References

  1. 1.
    West SA, Griffin AS, Gardner A et al (2006) Social evolution theory for microorganisms. Nat Rev Microbiol 4:597–607CrossRefGoogle Scholar
  2. 2.
    Wintermute EH, Silver PA (2010) Dynamics in the mixed microbial concourse. Genes Dev 24:2603–2614CrossRefGoogle Scholar
  3. 3.
    Marx CJ (2009) Microbiology. Getting in touch with your friends. Science 324:1150–1151CrossRefGoogle Scholar
  4. 4.
    Schink B (2002) Synergistic interactions in the microbial world. Antonie Van Leeuwenhoek 81:257–261CrossRefGoogle Scholar
  5. 5.
    Pettit RK (2009) Mixed fermentation for natural product drug discovery. Appl Microbiol Biotechnol 83:19–25CrossRefGoogle Scholar
  6. 6.
    Zuroff TR, Curtis WR (2012) Developing symbiotic consortia for lignocellulosic biofuel production. Appl Microbiol Biotechnol 93:1423–1435CrossRefGoogle Scholar
  7. 7.
    McInerney MJ, Struchtemeyer CG, Sieber J et al (2008) Physiology, ecology, phylogeny, and genomics of microorganisms capable of syntrophic metabolism. Ann N Y Acad Sci 1125:58–72CrossRefGoogle Scholar
  8. 8.
    Smid EJ, Lacroix C (2013) Microbe-microbe interactions in mixed culture food fermentations. Curr Opin Biotechnol 24:148–154CrossRefGoogle Scholar
  9. 9.
    Vartoukian SR, Palmer RM, Wade WG (2010) Strategies for culture of “unculturable” bacteria. FEMS Microbiol Lett 309:1–7Google Scholar
  10. 10.
    Brune KD, Bayer TS (2012) Engineering microbial consortia to enhance biomining and bioremediation. Front Microbiol 3:203CrossRefGoogle Scholar
  11. 11.
    Shou W, Ram S, Vilar JMG (2007) Synthetic cooperation in engineered yeast populations. Proc Natl Acad Sci U S A 104:1877–1882CrossRefGoogle Scholar
  12. 12.
    Hillesland KL, Stahl DA (2010) Rapid evolution of stability and productivity at the origin of a microbial mutualism. Proc Natl Acad Sci U S A 107:2124–2129CrossRefGoogle Scholar
  13. 13.
    Harcombe W (2010) Novel cooperation experimentally evolved between species. Evolution 64:2166–2172Google Scholar
  14. 14.
    Baba T, Ara T, Hasegawa M et al (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:2006.0008CrossRefGoogle Scholar
  15. 15.
    Umbarger HE (1969) Regulation of amino acid metabolism. Annu Rev Biochem 38:323–370CrossRefGoogle Scholar
  16. 16.
    Ishii K, Shiio I (1968) Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5′-phosphate dehydrogenase, xanthosine-5′-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis. J Biochem 63:661–669Google Scholar
  17. 17.
    Nierlich DP, Magasanik B (1965) Regulation of purine ribonucleotide synthesis by end product inhibition. The effect of adenine and guanine ribonucleotides on the 5′-phosphoribosyl-pyrophosphate amidotransferase of Aerobacter aerogenes. J Biol Chem 240:358–365Google Scholar
  18. 18.
    Lawrence DA, Smith DA, Rowbury RJ (1968) Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics 58:473–492Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Organismic and Evolutionary BiologyHarvard UniversityCambridgeUSA
  2. 2.Department of Ecology, Evolution, and BehaviorUniversity of MinnesotaSt. PaulUSA

Personalised recommendations