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Engineering and Analyzing Multicellular Systems pp 151–164Cite as

Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Membrane

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1151))

Abstract

The microbial coculture of multiple cell populations is used to study community evolution and for bioengineering applications. The cells in coculture undergo dynamic changes because of cell–cell and cell–environment interactions. Transcriptome analysis allows us to study the molecular basis of these changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.

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Acknowledgements

This work was supported in part by JSPS KAKENHI grant number 25650147 and the “Global COE Program” of the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Graduate School of Information Science and Technology, Osaka University, Yamadaoka 1-5, Suita, Osaka, 565-0871, Japan

    Kazufumi Hosoda & Tetsuya Yomo

  2. Graduate School of Information Science, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara, 630-0192, Japan

    Naoaki Ono

  3. RIKEN Quantitative Biology Center, Furuedai 6-2-3, Suita, Osaka, 565-0874, Japan

    Shingo Suzuki

  4. Graduate School of Frontier Bioscience, Osaka University, Yamadaoka 1-3, Suita, Osaka, 565-0871, Japan

    Tetsuya Yomo

  5. Exploratory Research for Advanced Technology, Yamadaoka 1-5, Suita, Osaka, 565-0871, Japan

    Tetsuya Yomo

Authors
  1. Kazufumi Hosoda
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  2. Naoaki Ono
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  3. Shingo Suzuki
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  4. Tetsuya Yomo
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Corresponding author

Correspondence to Tetsuya Yomo .

Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Science & Technology of China,, Hefei, Anhui, China

    Lianhong Sun

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

    Wenying Shou

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Hosoda, K., Ono, N., Suzuki, S., Yomo, T. (2014). Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Membrane. In: Sun, L., Shou, W. (eds) Engineering and Analyzing Multicellular Systems. Methods in Molecular Biology, vol 1151. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0554-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-0554-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0553-9

  • Online ISBN: 978-1-4939-0554-6

  • eBook Packages: Springer Protocols

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