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Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Membrane

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Part of the Methods in Molecular Biology book series (MIMB, volume 1151)

Abstract

The microbial coculture of multiple cell populations is used to study community evolution and for bioengineering applications. The cells in coculture undergo dynamic changes because of cell–cell and cell–environment interactions. Transcriptome analysis allows us to study the molecular basis of these changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.

Key words

Transcriptome analysis Microbial coculture Synthetic ecosystem Cell culture insert Membrane coculture 
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Notes

Acknowledgements

This work was supported in part by JSPS KAKENHI grant number 25650147 and the “Global COE Program” of the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Graduate School of Information Science and Technology, Osaka UniversitySuitaJapan
  2. 2.Graduate School of Information Science, Nara Institute of Science and TechnologyIkomaJapan
  3. 3.RIKEN Quantitative Biology CenterSuitaJapan
  4. 4.Graduate School of Frontier Bioscience, Osaka UniversitySuitaJapan
  5. 5.Exploratory Research for Advanced TechnologySuitaJapan

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