Abstract
Pseudomonas aeruginosa is an opportunistic human pathogen responsible for severe to deadly infections in patients suffering from cystic fibrosis, AIDS, undergoing immune suppressing therapies or suffering from severe burns. In the recent years there has been an increasing interest in exploring animal infection models that, to a certain extent, could mimic human infections. Here we describe the use of the larvae of the greater wax moth Galleria mellonella as a non-expensive, easy-to-use, and easy-to-obtain animal model to study P. aeruginosa infections.
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References
Hendrickson EL, Plotnikova J, Mahajan-Miklos S, Rahme LG, Ausubel FM (2001) Differential roles of the Pseudomonas aeruginosa PA14 rpoN gene in pathogenicity in plants, nematodes, insects, and mice. J Bacteriol 183:7126–7134
Jander G, Rahme LG, Ausubel FM (2000) Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J Bacteriol 182:3843–3845
Miyata S, Casey M, Frank DW, Ausubel FM, Drenkard E (2003) Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect Immun 71:2404–2413
Ryan RP, Lucey J, O’Donovan K, McCarthy Y, Yang L, Tolker-Nielsen T, Dow JM (2009) HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa. Environ Microbiol 11:1126–1136
Inglis RF, Gardner A, Cornelis P, Buckling A (2009) Spite and virulence in the bacterium Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 106:5703–5707
Mil-Homens D, Fialho AM (2012) A BCAM0223 mutant of Burkholderia cenocepacia is deficient in hemagglutination, serum resistance, adhesion to epithelial cells and virulence. PLoS One 7:e41747
Muller CM, Conejero L, Spink N, Wand ME, Bancroft GJ, Titball RW (2012) Role of RelA and SpoT in Burkholderia pseudomallei virulence and immunity. Infect Immun 80:3247–3255
Thomson EL, Dennis JJ (2012) A Burkholderia cepacia complex non-ribosomal peptide-synthesized toxin is hemolytic and required for full virulence. Virulence 3:286–298
Agnoli K, Schwager S, Uehlinger S, Vergunst A, Viteri DF, Nguyen DT, Sokol PA, Carlier A, Eberl L (2012) Exposing the third chromosome of Burkholderia cepacia complex strains as a virulence plasmid. Mol Microbiol 83:362–378
Soukup AA, Farnoodian M, Berthier E, Keller NP (2012) NosA, a transcription factor important in Aspergillus fumigatus stress and developmental response, rescues the germination defect of a laeA deletion. Fungal Genet Biol 49:857–865
Liu H, Gravelat FN, Chiang LY, Chen D, Vanier G, Ejzykowicz DE, Ibrahim AS, Nierman WC, Sheppard DC, Filler SG (2010) Aspergillus fumigatus AcuM regulates both iron acquisition and gluconeogenesis. Mol Microbiol 78:1038–1054
St Leger RJ, Screen SE, Shams-Pirzadeh B (2000) Lack of host specialization in Aspergillus flavus. Appl Environ Microbiol 66:320–324
Hoffmann JA (1995) Innate immunity of insects. Curr Opin Immunol 7:4–10
Brennan M, Thomas DY, Whiteway M, Kavanagh K (2002) Correlation between virulence of Candida albicans mutants in mice and Galleria mellonella larvae. FEMS Immunol Med Microbiol 34:153–157
Sambrook J, Fritsch EF, Maniatis T (2008) Molecular cloning: a laboratory manual, vol 2,1989 edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Nadal Jimenez P, Koch G, Papaioannou E, Wahjudi M, Krzeslak J, Coenye T, Cool RH, Quax WJ (2010) Role of PvdQ in Pseudomonas aeruginosa virulence under iron-limiting conditions. Microbiology 156:49–59
Acknowledgments
We gratefully acknowledge Rien Hoge for helpful discussions regarding Galleria infection assays. This research was partly funded by EU grant Antibiotarget MEST-CT-2005-020278 to G.K. and P.N.J.
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Koch, G., Nadal-Jimenez, P., Cool, R.H., Quax, W.J. (2014). Assessing Pseudomonas Virulence with Nonmammalian Host: Galleria mellonella . In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_52
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_52
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