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Gene Transfer: Conjugation

Part of the Methods in Molecular Biology book series (MIMB,volume 1149)


Conjugation is a gene transfer process in which a recipient bacterium receives DNA from a donor bacterium by cell-to-cell contact through conjugative pili. Conjugation is mediated by certain plasmids or transposons. Here, we describe plasmid conjugation.

Key words

  • Conjugation
  • Gene transfer
  • Conjugative plasmid
  • Helper plasmid
  • Donor/recipient bacterium
  • oriT
  • Tra
  • Mob
  • Mating pair

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Correspondence to Sophie Bleves .

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Sana, T.G., Laubier, A., Bleves, S. (2014). Gene Transfer: Conjugation. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols