Abstract
Conjugation is a gene transfer process in which a recipient bacterium receives DNA from a donor bacterium by cell-to-cell contact through conjugative pili. Conjugation is mediated by certain plasmids or transposons. Here, we describe plasmid conjugation.
Key words
- Conjugation
- Gene transfer
- Conjugative plasmid
- Helper plasmid
- Donor/recipient bacterium
- oriT
- Tra
- Mob
- Mating pair
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References
Zechner EL, Lang S, Schildbach JF (2012) Assembly and mechanisms of bacterial type IV secretion machines. Philos Trans R Soc Lond B Biol Sci 367:1073–1087
de Lorenzo V, Timmis KN (1994) Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol 235:386–405
Figurski DH, Helinski DR (1979) Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76:1648–1652
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Sana, T.G., Laubier, A., Bleves, S. (2014). Gene Transfer: Conjugation. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_3
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-0472-3
Online ISBN: 978-1-4939-0473-0
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