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Transposon Mutagenesis

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Pseudomonas Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we present a detailed protocol for using Himar1 mariner-based transposon mutagenesis to create mutant libraries in P. aeruginosa.

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Washington, 1959 NE Pacific St HSB K161, Seattle, WA, 98195, USA

    Hemantha D. Kulasekara

Authors
  1. Hemantha D. Kulasekara
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Corresponding author

Correspondence to Hemantha D. Kulasekara .

Editor information

Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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© 2014 Springer Science+Business Media New York

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Kulasekara, H.D. (2014). Transposon Mutagenesis. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_39

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_39

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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