Monitoring Lectin Interactions with Carbohydrates

Part of the Methods in Molecular Biology book series (MIMB, volume 1149)

Abstract

Protein–carbohydrate interactions are often involved in the first step of infection and Pseudomonas aeruginosa produces several proteins that are able to bind specifically to glycan epitopes present on host epithelia. The experimental approaches for studying protein–carbohydrate interaction have been inspired, with some adaptations, from those commonly used for protein–protein or protein–ligand interactions. A range of methods are described herein for detecting lectin activity, screening for monosaccharide or oligosaccharide specificity, determining the affinity of binding together with thermodynamics and kinetics parameters, and producing crystal of lectin–carbohydrate complexes for further structural studies.

Key words

Lectins Adhesins Glycans Interactions 

Notes

Acknowledgments

The authors acknowledge funding from ANR grants PA-Antiadh (ANR-09-JCJC-0047) and Glycoasterix (ANR-08-PCVI-0028). Support from GDR Pseudomonas and Association Vaincre la Mucoviscidose is also acknowledged.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM)UMR7255 CNRS-Aix Marseille Université, Institut de Microbiologie de la MéditerranéeMarseille Cédex 20France
  2. 2.Centre de Recherches sur les Macromolécules VégétalesUPR5301 CNRS, Université Joseph Fourier and ICMGGrenoble Cédex 09France

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