Cyanide Measurements in Bacterial Culture and Sputum

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1149)

Abstract

Cyanide is produced by a few bacterial species, including Pseudomonas aeruginosa, and it has a role in the opportunistic infections of this bacterium including in cystic fibrosis lung infections. We describe two methods for determining cyanide in culture and patient sputum samples. One uses an ion-selective electrode to provide a convenient, rapid method of cyanide quantitation in culture or sputum, and the second is a semiquantitative method using Feigl–Anger paper that is useful for screening large numbers of bacterial strains for cyanide production.

Key words

Ion-selective electrode Hydrogen cyanide HCN Quorum sensing Cyanogenesis 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Chandrika Goh Nair
    • 1
  • Ben Ryall
    • 2
  • Huw D. Williams
    • 3
  1. 1.Department of Life SciencesImperial College LondonLondonUK
  2. 2.School of Molecular Bioscience, University of SydneySydneyAustralia
  3. 3.Department of Life SciencesImperial College LondonLondonUK

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