Cyanide Measurements in Bacterial Culture and Sputum

  • Chandrika Goh Nair
  • Ben Ryall
  • Huw D. WilliamsEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1149)


Cyanide is produced by a few bacterial species, including Pseudomonas aeruginosa, and it has a role in the opportunistic infections of this bacterium including in cystic fibrosis lung infections. We describe two methods for determining cyanide in culture and patient sputum samples. One uses an ion-selective electrode to provide a convenient, rapid method of cyanide quantitation in culture or sputum, and the second is a semiquantitative method using Feigl–Anger paper that is useful for screening large numbers of bacterial strains for cyanide production.

Key words

Ion-selective electrode Hydrogen cyanide HCN Quorum sensing Cyanogenesis 


  1. 1.
    Blumer C, Haas D (2000) Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis. Arch Microbiol 173:170–177PubMedCrossRefGoogle Scholar
  2. 2.
    Ryall B, Lee X, Zlosnik JE, Hoshino S, Williams HD (2008) Bacteria of the Burkholderia cepacia complex are cyanogenic under biofilm and colonial growth conditions. BMC Microbiol 8:108PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    Ryall B, Mitchell H, Mossialos D, Williams HD (2009) Cyanogenesis by the entomopathogenic bacterium Pseudomonas entomophila. Lett Appl Microbiol 49:131–135PubMedCrossRefGoogle Scholar
  4. 4.
    Williams HD, Ziosnik JEA, Ryall B (2007) Oxygen, cyanide and energy generation in the cystic fibrosis pathogen Pseudomonas aeruginosa. Adv Microb Physiol 52:1–71PubMedCrossRefGoogle Scholar
  5. 5.
    Haas D, Defago G (2005) Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol 3:307–319PubMedCrossRefGoogle Scholar
  6. 6.
    Gallagher LA, Manoil C (2001) Pseudomonas aeruginosa PAO1 kills Caenorhabditis elegans by cyanide poisoning. J Bacteriol 183:6207–6214PubMedCentralPubMedCrossRefGoogle Scholar
  7. 7.
    Broderick KE, Chan A, Balasubramanian M, Feala J, Reed SL et al (2008) Cyanide produced by human isolates of Pseudomonas aeruginosa contributes to lethality in Drosophila melanogaster. J Infect Dis 197:457–464PubMedCrossRefGoogle Scholar
  8. 8.
    Goldfarb WB, Margraf H (1967) Cyanide production by Pseudomonas aeruginosa. Ann Surg 165:104–110PubMedCentralPubMedCrossRefGoogle Scholar
  9. 9.
    Ryall B, Davies JC, Wilson R, Shoemark A, Williams HD (2008) Pseudomonas aeruginosa, cyanide accumulation and lung function in CF and non-CF bronchiectasis patients. Eur Respir J 32:740–747PubMedCrossRefGoogle Scholar
  10. 10.
    Sanderson K, Wescombe L, Kirov SM, Champion A, Reid DW (2008) Bacterial cyanogenesis occurs in the cystic fibrosis lung. Eur Respir J 32:329–333PubMedCrossRefGoogle Scholar
  11. 11.
    Feigl F, Anger V (1966) Replacement of benzidine by copper ethylacetoacetate and tetra base as spot-test reagent for hydrogen cyanide and cyanogen. Analyst 91:282–284PubMedCrossRefGoogle Scholar
  12. 12.
    Castric PA (1983) Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels. Can J Microbiol 29:1344–1349PubMedCrossRefGoogle Scholar
  13. 13.
    Takos A, Lai D, Mikkelsen L, Abou Hachem M, Shelton D et al (2010) Genetic screening identifies cyanogenesis-deficient mutants of Lotus japonicus and reveals enzymatic specificity in hydroxynitrile glucoside metabolism. Plant Cell 22:1605–1619PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Zlosnik JE, Williams HD (2004) Methods for assaying cyanide in bacterial culture supernatant. Lett Appl Microbiol 38:360–365PubMedCrossRefGoogle Scholar
  15. 15.
    Zlosnik JE, Tavankar GR, Bundy JG, Mossialos D, O’Toole R et al (2006) Investigation of the physiological relationship between the cyanide-insensitive oxidase and cyanide production in Pseudomonas aeruginosa. Microbiology 152:1407–1415PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Chandrika Goh Nair
    • 1
  • Ben Ryall
    • 2
  • Huw D. Williams
    • 3
    Email author
  1. 1.Department of Life SciencesImperial College LondonLondonUK
  2. 2.School of Molecular Bioscience, University of SydneySydneyAustralia
  3. 3.Department of Life SciencesImperial College LondonLondonUK

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