Abstract
Optogenetic methods are gaining broad recognition. The zebrafish is particularly useful for these applications as a model vertebrate due to a unique combination of translucent embryos/larvae and efficient transgenesis. Here, we describe a zebrafish model of light-induced cardiac deficiency. Upon illumination with intense green light, the membrane-tethered photosensitizer protein KillerRed acts as a photoinducer of reactive oxygen species which in turn cause changes in heart rate and contractility in hearts that express this transgene.
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Acknowledgements
We thank the personnel at the Institute of Molecular and Cell Biology facilities for the maintenance of zebrafish lines. This work was supported by an Institute of Molecular and Cell Biology institutional grant from the Agency for Science, Technology, and Research (A*STAR) of Singapore and A*STAR-BMRC-Mindef grant (to V.K.).
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Teh, C., Korzh, V. (2014). In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein. In: Cambridge, S. (eds) Photoswitching Proteins. Methods in Molecular Biology, vol 1148. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0470-9_15
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DOI: https://doi.org/10.1007/978-1-4939-0470-9_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0470-9
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