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Experimental Approaches to Investigating the Vaginal Biofilm Microbiome

  • Marc M. BaumEmail author
  • Manjula Gunawardana
  • Paul Webster
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1147)

Abstract

Unraveling the complex ecology of the vaginal biofilm microbiome relies on a number of complementary techniques. Here, we describe the experimental approaches for studying vaginal microbial biofilm samples with a focus on specimen preparation for subsequent analysis. The techniques include fluorescence microscopy, fluorescence in situ hybridization, and scanning and transmission electron microscopy. Isolation of microbial DNA and RNA from these samples is covered along with a brief discussion of chemical analysis methods.

Key words

Vaginal microbiome Microbial biofilms Fluorescence microscopy Fluorescence in situ hybridization Scanning electron microscopy Transmission electron microscopy Genomic microbial DNA RNA Correlative microscopy 

Notes

Acknowledgment

Research reported in this publication was supported, in part, by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number R01AI100744. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Marc M. Baum
    • 1
    Email author
  • Manjula Gunawardana
    • 1
  • Paul Webster
    • 1
  1. 1.Department of ChemistryOak Crest Institute of SciencePasadenaUSA

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