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Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants and Efficacy of Sanitizers

  • Sara A. BurgessEmail author
  • Denise Lindsay
  • Steve H. Flint
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1147)

Abstract

In many environments, bacteria can attach to a surface and grow into multicellular structures, otherwise known as biofilms. Many systems for studying these biofilms in the laboratory are available. To study biofilms of the thermophilic bacilli in milk powder-manufacturing plants, standard laboratory biofilm techniques need to be adapted. The focus of this chapter is on techniques that can be used for growing and analyzing biofilms of thermophilic bacilli that are isolated from dairy processing plants. These techniques include laboratory methods as well as how to set up a pilot-scale experiment. The laboratory methods consist of a microtiter plate assay, which is used for strain selection, and the CDC reactor, which is used for testing sanitizers and antimicrobial surfaces. In dairy processing, if a new sanitizer or antimicrobial surface appears to be promising, it is useful to carry out pilot-scale experiments before introducing it to a manufacturing plant. We describe how to set up a pilot-scale experiment for testing the efficacy of sanitizers against the thermophilic bacilli.

Key words

Biofilm Thermophiles Milk Dairy Anoxybacillus Geobacillus Sanitizers 

References

  1. 1.
    Murphy PM, Lynch D, Kelly PM (1999) Growth of thermophilic spore forming bacilli in milk during the manufacture of low heat powders. Int J Dairy Technol 52:45–50CrossRefGoogle Scholar
  2. 2.
    Scott SA, Brooks JD, Rakonjac J et al (2007) The formation of thermophilic spores during the manufacture of whole milk powder. Int J Dairy Technol 60:109–117CrossRefGoogle Scholar
  3. 3.
    Flint SH, Palmer J, Bloemen K et al (2001) The growth of Bacillus stearothermophilus on stainless steel. J Appl Microbiol 90:151–157PubMedCrossRefGoogle Scholar
  4. 4.
    Seale RB, Flint SH, McQuillan AJ et al (2008) Recovery of spores from thermophilic dairy bacilli and effects of their surface characteristics on attachment to different surfaces. Appl Environ Microbiol 74:731–737PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Burgess SA, Brooks JD, Rakonjac J et al (2009) The formation of spores in biofilms of Anoxybacillus flavithermus. J Appl Microbiol 107:1012–1018PubMedCrossRefGoogle Scholar
  6. 6.
    Buckingham-Meyer K, Goeres DM, Hamilton MA (2007) Comparative evaluation of biofilm disinfectant efficacy tests. J Microbiol Methods 70:236–244PubMedCrossRefGoogle Scholar
  7. 7.
    Luppens SBI, Reij MW, van der Heijden RWL et al (2002) Development of a standard test to assess the resistance of Staphylococcus aureus biofilm cells to disinfectants. Appl Environ Microbiol 68:4194–4200PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    McCoy WF, Bryers JD, Robbins J et al (1981) Observations of fouling biofilm formation. Can J Microbiol 27:910–917PubMedCrossRefGoogle Scholar
  9. 9.
    British Standards Institution (1997) Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas. Test method and requirements (phase 2, step 1). British Standard BS EN 1276:1997Google Scholar
  10. 10.
    Lindsay D, von Holy A (1999) Different responses of planktonic and attached Bacillus subtilis and Pseudomonas fluorescens to sanitizer treatment. J Food Prot 62:368–379PubMedGoogle Scholar
  11. 11.
    International Organization for Standardization (2004) ISO Standard 18593:2004. Microbiology of food and animal feeding stuffs—horizontal methods for sampling techniques from surfaces using contact plates and swabsGoogle Scholar
  12. 12.
    Ronimus RS, Parker LE, Turner N et al (2003) A RAPD-based comparison of thermophilic bacilli from milk powders. Int J Food Microbiol 85:45–61PubMedCrossRefGoogle Scholar
  13. 13.
    Lindsay D, Brözel VS, Mostert JF et al (2002) Differential efficacy of a chlorine dioxide-containing sanitizer against single species and binary biofilms of a dairy-associated Bacillus cereus and a Pseudomonas fluorescens isolate. J Appl Microbiol 92:352–361PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sara A. Burgess
    • 1
    Email author
  • Denise Lindsay
    • 2
  • Steve H. Flint
    • 3
  1. 1.Institute of Food, Nutrition and Human Health, Massey University and Fonterra Research and Development CentrePalmerston NorthNew Zealand
  2. 2.Fonterra Research and Development CentrePalmerston NorthNew Zealand
  3. 3.Institute of Food, Nutrition and Human Health, Massey UniversityPalmerston NorthNew Zealand

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