Abstract
Any growth or bioconversion in biofilms is accompanied by the release of heat. The heat (in J) is tightly related to the stoichiometry of the respective process via law of Hess, and the heat production rate (in W or J/s) is additionally related to the process kinetics. This heat and the heat production rate can nowadays be measured by modern calorimetry with extremely high sensitivity. Flow-through calorimetry allows the measurement of bioprocesses in biofilms in real time, without the need of invasive sample preparation and disturbing of biofilm processes. Furthermore, it can be applied for long-term measurements and is even applicable to turbid media. Chip or miniaturized calorimeters have the additional advantages of extremely short thermal equilibration times and the requirement of very small amounts of media and chemicals. The precision of flow-through chip calorimeters (about 3 mW/L) allows the detection of early stages of biofilm development (about 105 bacteria cm−2).
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References
Flemming H-C (2003) Role and levels of real-time monitoring for successful anti-fouling strategies—an overview. Water Sci Technol 47:1–8
Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta 1016:328–332
Maskow T, Harms H (2006) Real time insights into bioprocesses using calorimetry: state of the art and potential. Eng Life Sci 6:66–277
Dubrunfaut M (1856) Note sur al chaleur et le travail mecanique produits lar la fermentation vineuse. C R Acad Sci 42:945–948
Hill AV (1911) A new form of differential microcalorimeter, for the estimation of heat production in physiological, bacteriological or ferment reactions. J Physiol 43:260–285
Weppen P, Ebens J, Müller BG et al (1991) On-line estimation of biological oxygen demand using direct calorimetry on surface attached microbial cultures. Thermochim Acta 193:135–143
Wentzien S, Sand W, Albertsen A et al (1994) Thiosulfate and tetrathionate degradation as well as biofilm generation by Thiobacillus intermedius and Thiobacillus versutus studied by microcalorimetry, HPLC, and ion-pair chromatography. Arch Microbiol 161:116–125
von Rège H, Sand W (1998) Evaluation of biocide efficacy by microcalorimetric determination of microbial activity in biofilms. J Microbiol Methods 33:227–235
Peitzsch M, Kiesel B, Harms H et al (2008) Real time analysis of Escherichia coli biofilms using calorimetry. Chem Eng Process 47:1000–1006
Buchholz F, Harms H, Maskow T (2010) Biofilm research using calorimetry—a marriage made in heaven? Biotechnol J 5:1339–1350
Buchholz F, Wolf A, Lerchner J et al (2010) Chip calorimetry for fast and reliable evaluation of bactericidal and bacteriostatic treatments of biofilms. Antimicrob Agents Chemother 54:312–319
Mariana F, Buchholz F, Lerchner J et al (2013) Chip-calorimetric monitoring of biofilm eradication with antibiotics provides mechanistic information. Int J Med Microbiol 303:158–165
Buchholz F, Lerchner J, Mariana F et al (2012) Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria. Biofouling 23:351–362
Lerchner J, Wolf A, Wolf G et al (2006) A new micro-fluidic chip calorimeter for biochemical applications. Thermochim Acta 445:144–150
Maskow T, Schubert T, Wolf A et al (2011) Potentials and limitations of miniaturized calorimeters for bioprocess monitoring. Appl Microbiol Biotechnol 92:55–66
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Morais, F.M., Buchholz, F., Maskow, T. (2014). Chip Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics, and Biological Agents. In: Donelli, G. (eds) Microbial Biofilms. Methods in Molecular Biology, vol 1147. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0467-9_19
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DOI: https://doi.org/10.1007/978-1-4939-0467-9_19
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