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Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1147))

Abstract

To assay in vitro antimicrobial activity of substances such as antibiotics or antiseptics, standard methods both in liquid and on solid media are available. These procedures cannot be adequate for testing antimicrobial-releasing or biocidal polymer systems.

This chapter is focused on the description of methods that the authors have developed to evaluate the antimicrobial activity of either antimicrobial agent-releasing polymers or biocidal polymers. These assays can be applied to different types of water-soluble or insoluble polymer matrices.

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References

  1. Kumar C, Anand S (1998) Significance of microbial biofilms in food industry: a review. Int J Food Microbiol 42:9–27

    Article  CAS  PubMed  Google Scholar 

  2. Block J, Haudidier K, Pasquin J et al (1993) Biofilm accumulation in drinking water distribution systems. Biofouling 6:333–343

    Article  CAS  Google Scholar 

  3. Francolini I, Donelli G (2010) Prevention and control of biofilm-based medical-device-related infections. FEMS Immunol Med Microbiol 59:227–238

    CAS  PubMed  Google Scholar 

  4. Donelli G, Francolini I, Piozzi A et al (2002) New polymer-antibiotic systems to inhibit bacterial biofilm formation: a suitable approach to prevent central venous catheter-associated infections. J Chemother 14:501–507

    Article  CAS  PubMed  Google Scholar 

  5. Piozzi A, Francolini I, Occhiaperti L et al (2004) Antimicrobial activity of polyurethanes coated with antibiotics: a new approach to the realization of medical devices exempt from microbial colonization. Int J Pharm 280:173–183

    Article  CAS  PubMed  Google Scholar 

  6. Darouiche RO, Berger DH, Khardori N et al (2005) Comparison of antimicrobial impregnation with tunneling of long-term central venous catheters: a randomized controlled trial. Ann Surg 242:193–200

    Article  PubMed Central  PubMed  Google Scholar 

  7. Francolini I, Norris P, Piozzi A et al (2004) Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces. Antimicrob Agents Chemother 48:4360–4365

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Crisante F, Francolini I, Bellusci M et al (2009) Antibiotic delivery polyurethanes containing albumin and polyallylamine nanoparticles. Eur J Pharm Sci 36:555–564

    Article  CAS  PubMed  Google Scholar 

  9. Schierholz JM, Rump A, Pulverer G (1997) New antiinfectious biomaterials. Ciprofloxacin containing polyurethanes as potential drug delivery systems to prevent foreign-body infections. Arzneimittelforschung 47:70–74

    CAS  PubMed  Google Scholar 

  10. Francolini I, D’Ilario L, Guaglianone E et al (2010) Polyurethane anionomers containing metal ions with antimicrobial properties: thermal, mechanical and biological characterization. Acta Biomater 6:3482–3490

    Article  CAS  PubMed  Google Scholar 

  11. Francolini I, Taresco V, Crisante F et al (2013) Water soluble usnic acid-polyacrylamide complexes with enhanced antimicrobial activity against Staphylococcus epidermidis. Int J Mol Sci 14:7356–7369

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Francolini I, Ruggeri V, Martinelli A et al (2006) Novel metal-polyurethane complexes with enhanced antimicrobial activity. Macromol Rapid Comm 27:233–237

    Article  CAS  Google Scholar 

  13. Woo GL, Yang ML, Yin HQ et al (2002) Biological characterization of a novel biodegradable antimicrobial polymer synthesized with fluoroquinolones. J Biomed Mater Res 59:35–45

    Article  CAS  PubMed  Google Scholar 

  14. Kenawy E, Worley SD, Broughton R (2007) The chemistry and applications of antimicrobial polymers: a state-of-the-art review. Biomacromolecules 8:1359–1384

    Article  CAS  Google Scholar 

  15. Acar J, Goldstein F (1996) Disk susceptibility test. In: Lorian V (ed) Antibiotics in laboratory medicine, 4th edn. Lippincott Williams & Wilkins, Baltimore, MD, pp 1–51

    Google Scholar 

  16. Baldassarri L, Donelli G, Gelosia A et al (1996) Purification and characterization of the staphylococcal slime-associated antigen and its occurrence among Staphylococcus epidermidis clinical isolates. Infect Immun 64:3410–3415

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Donelli G, Francolini I, Romoli D et al (2007) Synergistic activity of dispersin B and cefamandole nafate in the inhibition of staphylococcal biofilm growth on polyurethanes. Antimicrob Agents Chemother 51:2733–2740

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Cockerill F, Wikler M, Alder J et al (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved standard 9th edn. Clinical and Laboratory Standards Institute M07-A9 (32):1–63

    Google Scholar 

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Correspondence to Iolanda Francolini .

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Francolini, I., Piozzi, A., Donelli, G. (2014). Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices. In: Donelli, G. (eds) Microbial Biofilms. Methods in Molecular Biology, vol 1147. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0467-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-0467-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0466-2

  • Online ISBN: 978-1-4939-0467-9

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