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Biofilm Matrix-Degrading Enzymes

  • Jeffrey B. KaplanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1147)

Abstract

Polymeric substances such as proteins, polysaccharides, and DNA constitute a major component of the biofilm matrix. Enzymes that depolymerize and degrade these components are useful tools for investigating the composition and function of the biofilm matrix. This chapter provides a brief overview of the most commonly used biofilm matrix-degrading enzymes and presents examples of their applications in biofilm research.

Key words

Biofilm Extracellular DNA Dispersin B DNase Matrix PNAG Proteinase K 

Notes

Acknowledgement

The author thanks Eun Sun Lee (New Jersey Dental School) for help with Fig. 3. This work was supported by NIH grant AI097182 and by American University.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of BiologyAmerican UniversityWashingtonUSA

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