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Biofilm Matrix-Degrading Enzymes

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Book cover Microbial Biofilms

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1147))

Abstract

Polymeric substances such as proteins, polysaccharides, and DNA constitute a major component of the biofilm matrix. Enzymes that depolymerize and degrade these components are useful tools for investigating the composition and function of the biofilm matrix. This chapter provides a brief overview of the most commonly used biofilm matrix-degrading enzymes and presents examples of their applications in biofilm research.

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Acknowledgement

The author thanks Eun Sun Lee (New Jersey Dental School) for help with Fig. 3. This work was supported by NIH grant AI097182 and by American University.

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Authors and Affiliations

  1. Department of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA

    Jeffrey B. Kaplan

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  1. Jeffrey B. Kaplan
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Corresponding author

Correspondence to Jeffrey B. Kaplan .

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Editors and Affiliations

  1. Fondazione Santa Lucia IRCCS Microbial Biofilm Laboratory, Rome, Italy

    Gianfranco Donelli

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Kaplan, J.B. (2014). Biofilm Matrix-Degrading Enzymes. In: Donelli, G. (eds) Microbial Biofilms. Methods in Molecular Biology, vol 1147. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0467-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-0467-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0466-2

  • Online ISBN: 978-1-4939-0467-9

  • eBook Packages: Springer Protocols

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