In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development

  • Marion Girardot
  • Christine ImbertEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1147)


Fungi are able to grow as a single-species or a more complex biofilm attached to inert surfaces (catheters…) or tissues (lung…). This last form is a microbial niche which must be considered as a major risk factor of developing a human fungal infection. Nowadays, only a few therapeutic agents have been shown to be active against fungal biofilms in vitro and/or in vivo. So there is a real need to find new anti-biofilm molecules. Here we describe in detail some rapid, 96-well microtiter plate-based methods, for the screening of compounds with anti-biofilm activity against Candida spp. yeasts. Two approaches will be considered: prophylactic or curative effects of the tested compounds by producing biofilms on two supports – polystyrene well surfaces and catheter sections.

Key words

Biofilms Candida spp. Antifungal compounds Catheters Microtiter plates Fungal infections Colorimetric assay XTT 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Laboratory of ecology and biology of the interactions, Faculty of Medicine PharmacyUniversity of Poitiers, UMR CNRS 7267PoitiersFrance

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