Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors
The ability of many pathogenic bacteria to grow as a biofilm results in lower susceptibility to antibiotic treatments and to the host immune response, thus leading to the development of chronic infections. The understanding that biofilms can play an important role in bacterial virulence has prompted the search for inhibitors of biofilm development and of biofilm-related cellular processes. In this report, we present two examples of target-based microbiological screenings for antimicrobials endowed with anti-biofilm activity, aimed respectively at the inhibition of the signal molecule cyclic di-GMP and of quorum sensing.
Key wordsBiofilm Antimicrobials Signal molecules Quorum sensing Acyl-homoserine lactone Elastase c-di-GMP Diguanylate cyclase
We wish to thank Nadia Raffaelli for the protocol of the HPLC method for c-di-GMP determination and for critical reading. Work in P.L.’s laboratory has been supported by the Italian Foundation for Research on Cystic Fibrosis (project FFC#13/2009, with the contribution of Delegazione Novara and Delegazione Cosenza-2) and by the CHEM-PROFARMA-NET Research Program (Project RBPR05NWWC_004) from the Italian Ministry for University and Research.
Funding to L.L. has been provided by the Italian Foundation for Research on Cystic Fibrosis (projects FFC#14/2010 and FFC#13/2011, with the contribution of delegazione FFC di Vittoria-Ragusa, Delegazione FFC del Lago di Garda con Gruppi di Sostegno di Chivasso, dell’Isola Bergamasca e della Valpolicella, Delegazione FFC di Verbania e Antonio Guadagnin & Figlio).