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Herpes Simplex Virus Growth, Preparation, and Assay

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1144)

Abstract

In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.

Key words

Virus growth Plaque purification Plaque titration Growth curve Virus stock Gradient purification 

Notes

Acknowledgment

This work was supported by grants from the University of Ferrara, AIDS National Program and PRIN 2009.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB)University of FerraraFerraraItaly

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