Herpes Simplex Virus pp 19-29 | Cite as
Herpes Simplex Virus Growth, Preparation, and Assay
Protocol
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Abstract
In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.
Key words
Virus growth Plaque purification Plaque titration Growth curve Virus stock Gradient purificationNotes
Acknowledgment
This work was supported by grants from the University of Ferrara, AIDS National Program and PRIN 2009.
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