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Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1144))

Abstract

Resistance testing of antivirals to herpes simplex virus type 1 can be done by phenotypic and genotypic methods. The determination of a resistant phenotype is based on the calculation of inhibitory concentrations for the antiviral drug, which should be tested. The main advantage is a clear interpretation of laboratory findings, but the method is time consuming and a considerable experience is required for handling infectious virus. Genotypic resistance testing is based on the detection of resistance-related mutations in viral genes encoding the thymidine kinase and DNA polymerase by means of amplification and sequencing. This approach has the advantage of being faster, but only frameshift mutations and stops of translation can be interpreted without doubt and numerous amino acid substitutions are diagnostically less conclusive.

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Authors and Affiliations

  1. Institute of Virology and Antiviral Therapy, Jena University Clinic, Friedrich Schiller University of Jena, Hans-Knoell-Straße 2, 07745, Jena, Germany

    Andreas Sauerbrei & Kathrin Bohn

Authors
  1. Andreas Sauerbrei
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  2. Kathrin Bohn
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Corresponding author

Correspondence to Andreas Sauerbrei .

Editor information

Editors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zürich, Zürich, Switzerland

    Cornel Fraefel

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Sauerbrei, A., Bohn, K. (2014). Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-0428-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0427-3

  • Online ISBN: 978-1-4939-0428-0

  • eBook Packages: Springer Protocols

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