Abstract
DNA vaccination is an attractive vaccination method. First, the production of plasmid DNA as a vaccine is considerably more cheap and simple than the production of recombinant protein. Second, the expression cassette of DNA vaccines can readily be modified, making DNA vaccines highly flexible. Finally, in animal models, DNA vaccination is able to induce potent cellular immune responses.
Over the past decade, the focus in the DNA vaccination field has in large part moved from intramuscular immunization towards dermal administration. As a natural “porte d’entrée” for pathogens, the skin is rich in antigen-presenting cells, which are required for generating an efficient antigen-specific immune response.
This chapter describes a DNA vaccination protocol that utilizes a simple tattooing device for the dermal delivery of plasmid DNA. This technique, called DNA tattooing, is capable of generating high frequencies of antigen-reactive T cells in mice and macaques.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Nagata T, Aoshi T, Uchijima M et al (2004) Cytotoxic T-lymphocyte-, and helper T-lymphocyte-oriented DNA vaccination. DNA Cell Biol 23:93–106
van Rooij N, van Buuren MM, Philips D et al (2013) Tumor exome analysis reveals neoantigen-specific T-cell reactivity in an ipilimumab-responsive melanoma. J Clin Oncol 31:e439
Lu YC, Yao X, Li YF et al (2013) Mutated PPP1R3B is recognized by T cells used to treat a melanoma patient who experienced a durable complete tumor regression. J Immunol 190:6034–6042
Kanitakis J (2002) Anatomy, histology and immunohistochemistry of normal human skin. Eur J Dermatol 12:390–399
Kataru RP, Jung K, Jang C et al (2009) Critical role of CD11b+ macrophages and VEGF in inflammatory lymphangiogenesis, antigen clearance, and inflammation resolution. Blood 113:5650–5659
Shklovskaya E, Roediger B, Fazekas de St GB (2008) Epidermal and dermal dendritic cells display differential activation and migratory behavior while sharing the ability to stimulate CD4+ T cell proliferation in vivo. J Immunol 181:418–430
Stoecklinger A, Grieshuber I, Scheiblhofer S et al (2007) Epidermal langerhans cells are dispensable for humoral and cell-mediated immunity elicited by gene gun immunization. J Immunol 179:886–893
Stoitzner P, Tripp CH, Eberhart A et al (2006) Langerhans cells cross-present antigen derived from skin. Proc Natl Acad Sci U S A 103:7783–7788
Liard C, Munier S, Joulin-Giet A et al (2012) Intradermal immunization triggers epidermal Langerhans cell mobilization required for CD8 T-cell immune responses. J Invest Dermatol 132:615–625
Bins AD, Wolkers MC, van den Boom MD et al (2007) In vivo antigen stability affects DNA vaccine immunogenicity. J Immunol 179:2126–2133
Kis EE, Winter G, Myschik J (2012) Devices for intradermal vaccination. Vaccine 30:523–538
Bins AD, Jorritsma A, Wolkers MC et al (2005) A rapid and potent DNA vaccination strategy defined by in vivo monitoring of antigen expression. Nat Med 11:899–904
van den Berg JH, Nujien B, Beijnen JH et al (2009) Optimization of intradermal vaccination by DNA tattooing in human skin. Hum Gene Ther 20:181–189
Wolkers MC, Toebes M, Okabe M et al (2002) Optimizing the efficacy of epitope-directed DNA vaccination. J Immunol 168:4998–5004
Oosterhuis K, Aleyd E, Vrijland K et al (2012) Rational design of DNA vaccines for the induction of human papillomavirus type 16 E6- and E7-specific cytotoxic T-cell responses. Hum Gene Ther 23:1301–1312
van den Berg JH, Quaak SG, Beijnen JH et al (2010) Lipopolysaccharide contamination in intradermal DNA vaccination: toxic impurity or adjuvant? Int J Pharm 390:32–36
Toebes M, Rodenko B, Ovaa H et al (2009) Generation of peptide MHC class I monomers and multimers through ligand exchange. Curr Protoc Immunol 87:18.16.1–18.16.20
Bins AD, van Rheenen J, Jalink K et al (2007) Intravital imaging of fluorescent markers and FRET probes by DNA tattooing. BMC Biotechnol 7:2
Oosterhuis K, Ohlschlager P, van den Berg JH et al (2011) Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7. Int J Cancer 129:397–406
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
van den Berg, J.H., Oosterhuis, K., Schumacher, T.N.M., Haanen, J.B.A.G., Bins, A.D. (2014). Intradermal Vaccination by DNA Tattooing. In: Rinaldi, M., Fioretti, D., Iurescia, S. (eds) DNA Vaccines. Methods in Molecular Biology, vol 1143. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0410-5_9
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0410-5_9
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0409-9
Online ISBN: 978-1-4939-0410-5
eBook Packages: Springer Protocols