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Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins

  • Nataliya Golovyashkina
  • Frederik Sündermann
  • Roland Brandt
  • Lidia Bakota
Protocol
Part of the Neuromethods book series (NM, volume 87)

Abstract

Dendritic morphology and its alterations determine the strength of the integrated signal of individual neurons. Changes in the dendritic arborization accompany neuronal development, memory formation, and neurodegenerative processes. Region-specific dendritic simplification is a key feature in Alzheimer’s disease and other neurodegenerative disorders. Here we describe a method to analyze whole-neuron morphology in brain slices prepared from transgenic mice expressing enhanced green fluorescent protein (EGFP) in different subpopulations of neurons. Complete pyramidal neurons are imaged using high-resolution confocal microscopy and analyzed after semi-automated 3D reconstruction. The approach allows evaluation of different morphological features, such as total extent of dendrites and number of branching points, as well as 3D Sholl analysis, e.g., of apical and basal dendritic subtrees of neurons from different hippocampal or cortical subregions.

Key words

Dendritic morphology Hippocampus Cortex Brain slices Laser scanning microscopy Semi-automated analysis 

Notes

Acknowledgements

The authors acknowledge the support given by the Deutsche Forschungsgemeinschaft (DFG grant BR1192/11-2) to R.B. and a Lichtenberg Fellowship of the state of Lower Saxony to F.S.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nataliya Golovyashkina
    • 1
  • Frederik Sündermann
    • 1
  • Roland Brandt
    • 1
  • Lidia Bakota
    • 1
  1. 1.Department of NeurobiologyUniversity of OsnabrückOsnabrückGermany

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