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Protein Structure Prediction pp 147–162Cite as

Prediction of Intrinsic Disorder in Proteins Using MFDp2

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1137))

Abstract

Intrinsically disordered proteins (IDPs) are either entirely disordered or contain disordered regions in their native state. IDPs were found to be abundant across all kingdoms of life, particularly in eukaryotes, and are implicated in numerous cellular processes. Experimental annotation of disorder lags behind the rapidly growing sizes of the protein databases and thus computational methods are used to close this gap and to investigate the disorder. MFDp2 is a novel webserver for accurate sequence-based prediction of protein disorder which also outputs well-described sequence-derived information that allows profiling the predicted disorder. We conveniently visualize sequence conservation, predicted secondary structure, relative solvent accessibility, and alignments to chains with annotated disorder. The webserver allows predictions for multiple proteins at the same time, includes help pages and tutorial, and the results can be downloaded as text-based (parsable) file. MFDp2 is freely available at http://biomine.ece.ualberta.ca/MFDp2/.

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Acknowledgments

This work was supported by the Dissertation fellowship awarded by the University of Alberta to M.J.M. and by the Discovery grant from the Natural Sciences and Engineering Research Council of Canada to L.K.

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Authors and Affiliations

  1. Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada

    Marcin J. Mizianty & Lukasz Kurgan

  2. Department of Molecular Medicine, Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA

    Vladimir Uversky

  3. Institute for Biological Instrumentation, Russian Academy of Sciences, Moscow Region, Russia

    Vladimir Uversky

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  1. Marcin J. Mizianty
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  2. Vladimir Uversky
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  3. Lukasz Kurgan
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  1. Purdue University Dept. Biological Science, West Lafayette, Indiana, USA

    Daisuke Kihara

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Mizianty, M.J., Uversky, V., Kurgan, L. (2014). Prediction of Intrinsic Disorder in Proteins Using MFDp2. In: Kihara, D. (eds) Protein Structure Prediction. Methods in Molecular Biology, vol 1137. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0366-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-0366-5_11

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