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Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High-Content Imaging

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Dengue

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1138))

Abstract

Large-scale screening of antiviral compounds that target dengue virus life cycle requires a robust cell-based assay that is rapid, easy to conduct, and sensitive enough to be able to assess viral infectivity and cell viability so that antiviral efficacy can be measured. In this chapter we describe a method that uses high-content imaging to evaluate the in vitro antiviral efficacy in a modification to the cell-based flavivirus immunodetection (CFI) assay that was described previously in Wang et al. (Antimicrob Agents Chemother 53(5):1823–1831, 2009).

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References

  1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496(7446):504–507

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. World Health Organization (2009) Dengue guidelines for diagnosis, treatment, prevention and control. http://www.who.int/rpc/guidelines/9789241547871/en/

  3. Whitehead SS, Blaney JE, Durbin AP, Murphy BR (2007) Prospects for a dengue virus vaccine. Nat Rev Microbiol 5(7):518–528

    Article  CAS  PubMed  Google Scholar 

  4. Roehrig JT, Hombach J, Barrett AD (2008) Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. Viral Immunol 21(2):123–132

    Article  CAS  PubMed  Google Scholar 

  5. Green N, Ott RD, Isaacs RJ, Fang H (2008) Cell-based assays to identify inhibitors of viral disease. Expert Opin Drug Discov 3(6): 671–676

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Noah JW, Severson W, Noah DL, Rasmussen L, White EL, Jonsson CB (2007) A cell-based luminescence assay is effective for high-throughput screening of potential influenza antivirals. Antiviral Res 73(1):50–59

    Article  CAS  PubMed  Google Scholar 

  7. Wang QY, Patel SJ, Vangrevelinghe E, Xu HY, Rao R, Jaber D, Schul W, Gu F, Heudi O, Ma NL, Poh MK, Phong WY, Keller TH, Jacoby E, Vasudevan SG (2009) A small-molecule dengue virus entry inhibitor. Antimicrob Agents Chemother 53(5):1823–1831

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Rathore AP, Paradkar PN, Watanabe S, Tan KH, Sung C, Connolly JE, Low J, Ooi EE, Vasudevan SG (2011) Celgosivir treatment misfolds dengue virus NS1 protein, induces cellular pro-survival genes and protects against lethal challenge mouse model. Antiviral Res 92(3):453–460

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Medical Research Council, Singapore (www.nmrc.gov.sg), under grant NMRC/1315/2011 to SGV and a Duke-NUS Graduate Medical School start-up grant to MK and SGV.

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Authors and Affiliations

  1. Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, Singapore, Singapore

    Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan & Manoj Krishnan

Authors
  1. Kah Hin Tan
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  2. Kitti Chan Wing Ki
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  3. Satoru Watanabe
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  4. Subhash G. Vasudevan
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  5. Manoj Krishnan
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Editor information

Editors and Affiliations

  1. Georgetown University Medical Center Dept. of Microbiology and Immunology, Washington, District of Columbia, USA

    Radhakrishnan Padmanabhan

  2. Duke-NUS GMS Singapore Emerging Infectious Diseases, Singapore, Singapore

    Subhash G. Vasudevan

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© 2014 Springer Science+Business Media, LLC

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Tan, K.H., Ki, K.C.W., Watanabe, S., Vasudevan, S.G., Krishnan, M. (2014). Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High-Content Imaging. In: Padmanabhan, R., Vasudevan, S. (eds) Dengue. Methods in Molecular Biology, vol 1138. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0348-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-0348-1_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0347-4

  • Online ISBN: 978-1-4939-0348-1

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