Abstract
Dendritic cells (DCs) are critical to the initiation of a T-cell response. They constitute the most potent antigen-presenting cell (APC) endowed with the unique capacity to stimulate an antigen-specific T-cell responses by naïve T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile clinically. Further, Ad vectors provide a high level of transgene expression, and Ad-transduced DCs can effectively present antigenic proteins. In this chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.
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Acknowledgement
The authors would like to thank Richard E. Neubiser, Afc, for his contributions to this chapter and Alice S. Cole for her assistance in with manuscript preparation.
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Senesac, J., Gabrilovich, D., Pirruccello, S., Talmadge, J.E. (2014). Dendritic Cells Transfected with Adenoviral Vectors as Vaccines. In: Lawman, M., Lawman, P. (eds) Cancer Vaccines. Methods in Molecular Biology, vol 1139. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0345-0_10
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DOI: https://doi.org/10.1007/978-1-4939-0345-0_10
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