Dendritic Cells Transfected with Adenoviral Vectors as Vaccines

  • Joseph Senesac
  • Dmitry Gabrilovich
  • Samuel Pirruccello
  • James E. Talmadge
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1139)

Abstract

Dendritic cells (DCs) are critical to the initiation of a T-cell response. They constitute the most potent antigen-presenting cell (APC) endowed with the unique capacity to stimulate an antigen-specific T-cell responses by naïve T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile clinically. Further, Ad vectors provide a high level of transgene expression, and Ad-transduced DCs can effectively present antigenic proteins. In this chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.

Key words

Monocytes Dendritic cells Adenovirus Vaccine Release assays 

Notes

Acknowledgement

The authors would like to thank Richard E. Neubiser, Afc, for his contributions to this chapter and Alice S. Cole for her assistance in with manuscript preparation.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Joseph Senesac
    • 1
  • Dmitry Gabrilovich
    • 2
  • Samuel Pirruccello
    • 3
  • James E. Talmadge
    • 3
  1. 1.Bellicum PharmaceuticalsHoustonUSA
  2. 2.H. Lee Moffitt Cancer CenterUniversity of South FloridaTampaUSA
  3. 3.University of Nebraska Medical CenterOmahaUSA

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