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Plant Epigenetics pp 225–236Cite as

Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1456))

Abstract

Virus-induced gene silencing (VIGS) is a powerful epigenetic tool that allows in a relatively short period of time to down-regulate the expression of an endogenous gene in infected plants for either monitoring the resulting phenotype or enhancing/modifying a particular trait associated with the gene. Here, we describe the utilization of Tobacco rattle virus (TRV) as a vector for the VIGS technique in Arabidopsis plants. The unique ability of TRV to infect both somatic tissues and gametes allows deciphering the role of genes in these tissues simultaneously. As an example, we demonstrate the utilization of TRV to down-regulate the expression of AGO2 and NRPD1a genes in ovules of Arabidopsis plants in order to boost the stable transformation efficiency by floral dip.

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Acknowledgment

We acknowledge the grants from the Natural Sciences and Engineering Research Council of Canada and Agricultural Research Consortium of Alberta. We thank Nina Kepeshchuk for technical assistance and Valentina Titova for proofreading the manuscript.

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Authors and Affiliations

  1. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Andriy Bilichak

  2. Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4

    Igor Kovalchuk

Authors
  1. Andriy Bilichak
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  2. Igor Kovalchuk
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Corresponding author

Correspondence to Igor Kovalchuk .

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Editors and Affiliations

  1. Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada

    Igor Kovalchuk

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Bilichak, A., Kovalchuk, I. (2017). Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing. In: Kovalchuk, I. (eds) Plant Epigenetics. Methods in Molecular Biology, vol 1456. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-4899-7708-3_17

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  • DOI: https://doi.org/10.1007/978-1-4899-7708-3_17

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  • Publisher Name: Humana Press, Boston, MA

  • Print ISBN: 978-1-4899-7706-9

  • Online ISBN: 978-1-4899-7708-3

  • eBook Packages: Springer Protocols

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