Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells

  • Isabel Siegert
  • Valentin Schatz
  • Alexander T. Prechtel
  • Alexander Steinkasserer
  • Christian Bogdan
  • Jonathan Jantsch
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)


Dendritic cells (DC) and macrophages (MΦ) play a pivotal role in antimicrobial defense, in the regulation of immune responses, and in maintaining tissue homeostasis. The analysis of DC and MΦ function relies on primary cells albeit these cells are known to be difficult to transfect. This makes the use of small interfering RNA (siRNA) for targeted manipulation of gene expression by RNA interference difficult. In the following chapter, we provide a detailed protocol for the successful transfer of siRNA via electroporation into a defined population of mouse bone marrow-derived MΦ or DC that does not cause toxicity to the myeloid cells or nonspecific alterations of their biological functions. Factors that influence the transfection and knockdown rate will be highlighted.

Key words

siRNA Electroporation Dendritic cells Macrophages Gene expression 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Isabel Siegert
    • 1
  • Valentin Schatz
    • 1
  • Alexander T. Prechtel
    • 2
  • Alexander Steinkasserer
    • 3
  • Christian Bogdan
    • 1
  • Jonathan Jantsch
    • 1
  1. 1.Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und HygieneUniversitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany
  2. 2.Boehringer Ingelheim Pharma GmbH und Co. KGBiberach an der RißGermany
  3. 3.Abteilung Immunmodulation, HautklinikUniversitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen- NürnbergErlangenGermany

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