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Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips

  • Zewen Wei
  • Zhihong Li
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Abstract

Electroporation is a high-efficiency and low-toxicity physical gene transfer method. Traditional electroporation is limited to only low volume cell samples. Here we present a continuous cell electroporation method based on commonly used microfluidic chip fabrication technology. Using easily fabricated PDMS microfluidic chip, syringe pumps, and pulse generator, we show efficient delivery of both DNA and siRNA into different cell lines. We describe the protocol of chip fabrication, apparatus setup, and cell electroporation assay. Typically, the fabrication of the devices takes 1 or 2 days and the continuous electroporation assay takes 1 h.

Key words

Electroporation Microfluidic Gene delivery Laminar flow 

Notes

Acknowledgment

This work was supported by the National Natural Science Foundation of China (61176111 and 61204118).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Zewen Wei
    • 1
  • Zhihong Li
    • 2
  1. 1.National Center for Nanoscience and TechnologyBeijingChina
  2. 2.National Key Laboratory of Science and Technology on Micro/Nano FabricationInstitute of Microelectronics, Peking UniversityBeijingChina

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