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The Impact of Non-electrical Factors on Electrical Gene Transfer

  • Jiemiao Hu
  • Jeffry Cutrera
  • Shulin Li
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Abstract

Electrical pulses directly and effectively boost both in vitro and in vivo gene transfer, but this process is greatly affected by non-electrical factors that exist during electroporation. These factors include, but are not limited to, the types of cells or tissues used, property of DNA, DNA formulation, and expressed protein. In this mini-review, we only describe and discuss a summary of DNA properties and selected DNA formulations on gene transfer via electroporation. The properties of DNA were selected for review because a substantial amount of remarkable work has been performed during the past few years but has received less notice than other works, although DNA properties appear to be critical for boosting electroporation delivery. The selected formulations will be covered in this mini-review because we are only interested in the simple formulations that could be used for cell or gene therapy via electroporation. Plus, there was an extensive review of DNA formulations in the first edition of this book. The formulations discussed in this mini-review represent novel developments in recent years and may impact electroporation significantly. These advancements in DNA formulations could prove to be important for gene delivery and disease treatment.

Key words

Non-electrical factors DNA properties DNA formulation Electrical gene transfer 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jiemiao Hu
    • 1
    • 2
  • Jeffry Cutrera
    • 1
    • 2
  • Shulin Li
    • 1
  1. 1.Department of PediatricsThe University of Texas MD Anderson Cancer CenterHoustonUSA
  2. 2.Department of Musculoskeletal Oncology, Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina

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