Abstract
Strategies to improve vaccine efficacy are still required. The immunogenicity of DNA vaccines is strongly improved by electroporation (EP). The skin is populated with a wide variety of immune cells, making it an attractive tissue for vaccine delivery. Here we describe a method for the EP-mediated intradermal delivery of DNA vaccines in nonhuman primates, as a model for preclinical development of human vaccines, using noninvasive needleless electrodes.
Key words
- DNA
- Vaccine
- Electroporation
- Skin
- Nonhuman primate
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Adam, L., Le Grand, R., Martinon, F. (2014). Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_27
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DOI: https://doi.org/10.1007/978-1-4614-9632-8_27
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Publisher Name: Humana Press, New York, NY
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