In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart

  • Hendrik T. Tevaearai
  • Amiq Gazdhar
  • Marie-Noëlle Giraud
  • Martin Flück
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)


Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. Here, we describe two protocols involving in vivo electroporation for gene transfer to the beating heart.

Key words

Gene transfer Electroporation Heart 



This work was supported by the Olga Mayenfisch Foundation Zürich, Switzerland, and two grants from the Swiss National Science Foundation (3200-068304 and 31000-118270/1). The authors would like to thank Mrs Laura Seidel for English and administrative assistance.


  1. 1.
    Roger VL, Go AS, Lloyd-Jones DM et al (2011) Heart disease and stroke statistics-2011 update: a report from the American Heart Association. Circulation 123:e18–e209PubMedCrossRefGoogle Scholar
  2. 2.
    Katz MG, Swain JD, White JD et al (2010) Cardiac gene therapy: optimization of gene delivery techniques in vivo. Hum Gene Ther 21:371–380PubMedCrossRefGoogle Scholar
  3. 3.
    Jaski BE, Jessup ML, Mancini DM et al (2009) Calcium upregulation by percutaneous administration of gene therapy in cardiac disease (CUPID Trial), a first-inhuman phase 1/2 clinical trial. J Card Fail 15:171–181PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Njeim MT, Hajjar RJ (2010) Gene therapy for heart failure. Arch Cardiovasc Dis 103: 477–485PubMedCrossRefGoogle Scholar
  5. 5.
    Gazdhar A, Bilici M, Pierog J et al (2006) In vivo electroporation and ubiquitin promoter – a protocol for sustained gene expression in the lung. J Gene Med 8:910–918PubMedCrossRefGoogle Scholar
  6. 6.
    Gissel H (2010) Effects of varying pulse parameters on ion homeostasis, cellular integrity, and force following electroporation of rat muscle in vivo. Am J Physiol Regul Integr Comp Physiol 298:R918–R929PubMedCrossRefGoogle Scholar
  7. 7.
    Durieux AC, Bonnefoy R, Manissolle C et al (2002) High-efficiency gene electrotransfer into skeletal muscle: description and physiological applicability of a new pulse generator. Biochem Biophys Res Commun 296:443–450PubMedCrossRefGoogle Scholar
  8. 8.
    Mathiesen I (1999) Electropermeabilization of skeletal muscle enhances gene transfer in vivo. Gene Ther 6:508–514PubMedCrossRefGoogle Scholar
  9. 9.
    Mir LM, Bureau MF, Gehl J et al (1999) High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci U S A 96:4262–4267PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Harrison RL, Byrne BJ, Tung L (1998) Electroporation-mediated gene transfer in cardiac tissue. FEBS Lett 435:1–5PubMedCrossRefGoogle Scholar
  11. 11.
    Wang Y, Bai Y, Price C et al (2001) Combination of electroporation and DNA/dendrimer complexes enhances gene transfer into murine cardiac transplants. Am J Transplant 1:334–338PubMedCrossRefGoogle Scholar
  12. 12.
    Marshall WG Jr, Boone BA, Burgos JD et al (2010) Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression. Gene Ther 17:419–423PubMedCentralPubMedCrossRefGoogle Scholar
  13. 13.
    Ayuni EL, Gazdhar A, Giraud MN et al (2010) In vivo electroporation mediated gene delivery to the beating heart. PLoS One 5, E14467PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Eigeldinger-Berthou S, Buntschu P, Flück M et al (2012) Electric pulses augment reporter gene expression in the beating heart. J Gene Med 14:191–203PubMedCrossRefGoogle Scholar
  15. 15.
    Davalos RV, Rubinsky B, Mir LM (2003) Theoretical analysis of the thermal effects during in vivo tissue electroporation. Bioelectrochemistry 61:99–107PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hendrik T. Tevaearai
    • 1
  • Amiq Gazdhar
    • 2
  • Marie-Noëlle Giraud
    • 3
  • Martin Flück
    • 4
  1. 1.Department of Cardiovascular SurgeryInselspital, Berne University HospitalBerneSwitzerland
  2. 2.Division of Pulmonary MedicineBerne University Hospital, University of BerneBerneSwitzerland
  3. 3.Department of CardiologyUniversity Hospital FribourgFribourgSwitzerland
  4. 4.Laboratory for Muscle PlasticityBalgrist University Hospital, University of ZurichZurichSwitzerland

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