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In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart

  • Hendrik T. Tevaearai
  • Amiq Gazdhar
  • Marie-Noëlle Giraud
  • Martin Flück
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Abstract

Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. Here, we describe two protocols involving in vivo electroporation for gene transfer to the beating heart.

Key words

Gene transfer Electroporation Heart 

Notes

Acknowledgements

This work was supported by the Olga Mayenfisch Foundation Zürich, Switzerland, and two grants from the Swiss National Science Foundation (3200-068304 and 31000-118270/1). The authors would like to thank Mrs Laura Seidel for English and administrative assistance.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hendrik T. Tevaearai
    • 1
  • Amiq Gazdhar
    • 2
  • Marie-Noëlle Giraud
    • 3
  • Martin Flück
    • 4
  1. 1.Department of Cardiovascular SurgeryInselspital, Berne University HospitalBerneSwitzerland
  2. 2.Division of Pulmonary MedicineBerne University Hospital, University of BerneBerneSwitzerland
  3. 3.Department of CardiologyUniversity Hospital FribourgFribourgSwitzerland
  4. 4.Laboratory for Muscle PlasticityBalgrist University Hospital, University of ZurichZurichSwitzerland

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