Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion

  • David Machado-Aranda
  • Krishnan Raghavendran
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)


Several of the biological processes involved in the pathogenesis of acute lung injury and acute respiratory distress syndrome after lung contusion are regulated at a genetic and epigenetic level. Thus, strategies to manipulate gene expression in this context are highly desirable not only to elucidate the mechanisms involved but also to look for potential therapies. In the present chapter, we describe mouse and rat models of inducing blunt thoracic injury followed by electroporation-mediated gene delivery to the lung. Electroporation is a highly efficient and easily reproducible technique that allows circumvention of several of lung gene delivery challenges and safety issues present with other forms of lung gene therapy.

Key words

Lung contusion Acute lung injury Acute respiratory distress syndrome Electroporation 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • David Machado-Aranda
    • 1
  • Krishnan Raghavendran
    • 1
  1. 1.Division of Acute Care Surgery, Department of SurgeryUniversity of MichiganAnn ArborUSA

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