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Electroporation-Mediated Gene Delivery to the Lungs

  • Jennifer L. Young
  • Michael S. Barravecchia
  • David A. Dean
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Abstract

Electroporation is a safe, efficient, and inexpensive method to transfer naked plasmid DNA into various tissues. For electroporation-mediated gene transfer to the mouse lung, a plasmid solution is delivered to the lungs via the trachea. Immediately after plasmid delivery, eight square wave pulses are delivered by two pre-gelled electrodes placed on each side of the chest. An optimal field strength in mice is 200 V/cm, with a pulse duration of 10 ms each and a 1 s interval between pulses. High level gene expression can be achieved within 24 h in all cell types in the lung with very little inflammation and no apparent trauma.

Key words

Electroporation Nonviral gene therapy Plasmid Lungs Whole lung tissue extraction Bronchoalveolar lavage fluid (BALF) Intubation 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jennifer L. Young
    • 1
  • Michael S. Barravecchia
    • 1
  • David A. Dean
    • 1
  1. 1.Division of Neonatology, Department of PediatricsSchool of Medicine and Dentistry, University of RochesterRochesterUSA

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