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Intradermal Electroporation of RNA

  • Maria L. Knudsen
  • Karl Ljungberg
  • Peter Liljeström
  • Daniel X. Johansson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1121)

Abstract

This chapter describes the in vivo delivery of conventional mRNA or alphaviral replicon RNA via intradermal electroporation. The use of RNA in clinical applications has several potential advantages compared to DNA. For instance, RNA cannot integrate into the host genome, and it does not contain bacterial sequence motifs such as CpG often present in plasmid DNA backbones that can potentially trigger autoimmune responses. Intradermal electroporation is well tolerated and causes only minor trauma compared to intramuscular electroporation. As the skin houses high concentrations of antigen-presenting cells, vaccines could especially benefit from intradermal administration of RNA.

Key words

RNA Intradermal Electroporation Alphavirus Semliki Forest virus Replicon 

Notes

Acknowledgements

This project was supported by the Swedish Research Council and Swedish International Development Cooperation Agency.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Maria L. Knudsen
    • 1
  • Karl Ljungberg
    • 1
  • Peter Liljeström
    • 1
  • Daniel X. Johansson
    • 1
  1. 1.Department of Microbiology, Tumor and Cell BiologyKarolinska InstitutetStockholmSweden

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