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Bone Marrow and Stem Cell Transplantation pp 115–138Cite as

Molecular Typing Methods for Minor Histocompatibility Antigens

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1109))

Abstract

Minor histocompatibility (H) antigen mismatching leads to clinically relevant alloimmune reactivity. Depending on the tissue expression pattern of the involved minor H antigens, the immune response may either cause graft-versus-host disease and a graft-versus-tumor effect or lead to only a graft-versus-leukemia effect. Thus, identification of recipient–donor pairs with minor H antigen mismatches has clinical importance. This chapter describes molecular typing methods for molecular typing of minor H antigens.

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Acknowledgments

Talitha de Hoop and Walter van Ginkel, University Medical Center Utrecht, are acknowledged for developing and validating the SBT protocols; Jos Pool and Jos Drabbels, Leiden University Medical Center, for developing and validating the uniform PCR-SSP protocols; and Erik Rozemuller, GenDx BV, Utrecht, for the implementation of automated allele assignment for minor H antigens after SBT in SBTEngine©.

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Authors and Affiliations

  1. Laboratory for Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands

    Eric Spierings

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  1. Eric Spierings
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  1. Department of Hematology, Ankara University, Ankara, Turkey

    Meral Beksaç

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Spierings, E. (2014). Molecular Typing Methods for Minor Histocompatibility Antigens. In: Beksaç, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Biology, vol 1109. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9437-9_8

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  • DOI: https://doi.org/10.1007/978-1-4614-9437-9_8

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  • Print ISBN: 978-1-4614-9436-2

  • Online ISBN: 978-1-4614-9437-9

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