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An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation

  • Katy Latham
  • Ann-Margaret Little
  • J. Alejandro Madrigal
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1109)

Abstract

The selection of a related or an unrelated hematopoietic stem cell donor for a patient requires accurate matching of human leukocyte antigen (HLA) genes in order to maximize the beneficial effects of the transplant. There are various different factors a laboratory must consider in order to achieve an HLA type including the number of samples being processed, level of resolution to be achieved, cost of providing the various tests, and turnaround time required. Each method has its advantages and disadvantages, and in most laboratories, a combination of methods may be used.

Key words

HLA typing PCR-SSP PCR-SSO PCR-SBT NGS 

Notes

Acknowledgments

Dr. Raymond Fernando and Mr. Franco Tavarozzi are thanked for providing Fig. 2.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Katy Latham
    • 1
  • Ann-Margaret Little
    • 2
  • J. Alejandro Madrigal
    • 1
  1. 1.Anthony Nolan Research InstituteUniversity College LondonLondonUK
  2. 2.Histocompatibility and Immunogenetics, Greater Glasgow and ClydeGartnavel General HospitalGlasgowUK

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