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Methods for Functional Analysis of Stem Cells

  • Michelle Escobedo-Cousin
  • J. Alejandro Madrigal
  • Aurore Saudemont
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1109)

Abstract

Hematopoietic stem cells (HSC) are rare, multipotent cells characterized by their ability to self-renew and to generate all blood cells throughout life. Major advances have been made in the area of HSC research as a result of the development of different techniques that allowed HSC identification, purification, and analysis of biological functions. This chapter presents methods that are currently used to analyze HSC functions in vitro based on their characteristics.

Key words

Hematopoietic stem cells Self-renewal Differentiation Reconstitution In vitro assays 

Notes

Acknowledgements

Research in the laboratory is funded by Anthony Nolan. M. Escobedo-Cousin is the recipient of a CONACyT and SEP fellowship.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Michelle Escobedo-Cousin
    • 1
  • J. Alejandro Madrigal
    • 1
  • Aurore Saudemont
    • 1
  1. 1.Anthony Nolan Research InstituteUniversity College LondonLondonUK

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