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Bone Marrow and Stem Cell Transplantation pp 23–46Cite as

Flow Cytometry for Hematopoietic Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1109))

Abstract

Within the last 25 years, flow cytometry and fluorescence-activated cell sorting have emerged as both routine diagnostic tools in clinical medicine and as advanced analytic tools critical in performing scientific research. This chapter aims at summarizing the use of flow cytometry in benign and malignant hematology and the monitoring of inherited and acquired immunodeficiency states. Numerous figures are provided from our laboratories at Massachusetts General Hospital that illustrate examples of these conditions. The chapter also describes novel flow cytometry-based imaging techniques, the combination of flow cytometry and mass spectrography, new software tools, and some future directions and applications of advanced instrumentation for flow cytometry.

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Acknowledgements

The authors would like to thank Laura Dillon, David Dombkowski, Abby Kelliher, and Scott Mordecai for their gracious help and specific contributions to this chapter.

Disclaimers The authors have no conflicts of interest to disclaim.

Source of support: K08 CA138916-02 to DSK and grants 1S10OD012027-01A1 and 1S10RR020936-01 to FIP.

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Authors and Affiliations

  1. Department of Pathology, Massachusetts General Hospital, Boston, MA, USA

    Daniela S. Krause, Michelle E. DeLelys & Frederic I. Preffer

Authors
  1. Daniela S. Krause
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  2. Michelle E. DeLelys
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  3. Frederic I. Preffer
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Editor information

Editors and Affiliations

  1. Department of Hematology, Ankara University, Ankara, Turkey

    Meral Beksaç

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Krause, D.S., DeLelys, M.E., Preffer, F.I. (2014). Flow Cytometry for Hematopoietic Cells. In: Beksaç, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Biology, vol 1109. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9437-9_2

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  • DOI: https://doi.org/10.1007/978-1-4614-9437-9_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9436-2

  • Online ISBN: 978-1-4614-9437-9

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