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Flow Cytometry for Hematopoietic Cells

  • Daniela S. Krause
  • Michelle E. DeLelys
  • Frederic I. Preffer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1109)

Abstract

Within the last 25 years, flow cytometry and fluorescence-activated cell sorting have emerged as both routine diagnostic tools in clinical medicine and as advanced analytic tools critical in performing scientific research. This chapter aims at summarizing the use of flow cytometry in benign and malignant hematology and the monitoring of inherited and acquired immunodeficiency states. Numerous figures are provided from our laboratories at Massachusetts General Hospital that illustrate examples of these conditions. The chapter also describes novel flow cytometry-based imaging techniques, the combination of flow cytometry and mass spectrography, new software tools, and some future directions and applications of advanced instrumentation for flow cytometry.

Key words

Flow cytometry Diagnostic immunophenotyping Hematopoietic malignancies Hematopathology Immunodeficiencies Hematopoietic stem cells Bone marrow transplantation Paroxysmal nocturnal hemoglobinuria Amnis imaging cytometry CyTOF mass cytometry Gemstone probability state model 

Notes

Acknowledgements

The authors would like to thank Laura Dillon, David Dombkowski, Abby Kelliher, and Scott Mordecai for their gracious help and specific contributions to this chapter.

Disclaimers The authors have no conflicts of interest to disclaim.

Source of support: K08 CA138916-02 to DSK and grants 1S10OD012027-01A1 and 1S10RR020936-01 to FIP.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Daniela S. Krause
    • 1
  • Michelle E. DeLelys
    • 1
  • Frederic I. Preffer
    • 1
  1. 1.Department of PathologyMassachusetts General HospitalBostonUSA

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