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Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders

  • Paolo Corradini
  • Cristiana Carniti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1109)

Abstract

Relapse represents the main cause of treatment failure after stem cell transplantation (SCT). Thus, monitoring of minimal residual disease (MRD) in allografted patients allows an early detection of recurrence and a subsequent intervention prior to clinically detectable relapse. MRD assessment by polymerase chain reaction-based methods is currently part of the routine clinical management of patients with chronic myeloid leukemia after allo-SCT. It is also recognized that it is a useful prognostic tool in several mature lymphoid and plasma cell disorders such as chronic lymphocytic leukemia, follicular lymphoma, mantle cell lymphoma, and multiple myeloma. In some of these entities, clinical trials employing MRD as a decision-making tool are currently ongoing and will define whether sensitive MRD detection allows for earlier therapeutic intervention to improve the outcome of SCT. We here discuss the methods of MRD evaluation in lymphoid and plasma cell disorders following transplantation with the ultimate aim of providing critical information for the setup of molecular approaches to detect MRD.

Key words

Minimal residual disease Stem cell transplantation Polymerase chain reaction Lymphoid malignancies Multiple myeloma 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Paolo Corradini
    • 1
  • Cristiana Carniti
    • 2
  1. 1.Division of Hematology, Fondazione IRCCS Istituto Nazionale Tumori MilanoUniversity of MilanoMilanItaly
  2. 2.Division of Hematology, Bone Marrow Transplantation Unit, Fondazione IRCCS Istituto Nazionale Tumori MilanoUniversity of MilanoMilanItaly

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