Adhesion of Tumor Cells to Matrices and Endothelium
Adhesion of tumor cells to matrix components and endothelial cells is essential for tumor metastasis. Investigation of the adhesion molecules required and the signals which induce tumor cell adhesion and migration are crucial in order to increase our understanding of this process. This chapter describes protocols which may be used to study tumor cell adhesion to purified matrix elements and tissue sections. It also details methods used to investigate cell adhesion to endothelial cells, both under static and flow conditions. In addition, there is a section detailing the use of endothelial cell cultures on three-dimensional collagen gels which are useful when studying adhesion to endothelial cells and onward invasion through a protein matrix.
Key wordsAdhesion Invasion Endothelial Migration Integrin Selectin
- 20.Rainger GE, Fisher A, Shearman C, Nash GB (1995) Adhesion of flowing neutrophils to cultured endothelial cells after hypoxia and reoxygenation in vitro. Am J Physiol Heart Circ Physiol 269:H1398–H1406Google Scholar
- 23.Lackie JM (1991) Adhesion from flow. In: Curtis ASG, Lackie JM (eds) Measuring cell adhesion. Wiley, New York, pp 41–65Google Scholar
- 24.Jones DA, Smith CW, McIntire L (1995) Effects of fluid shear stress on leukocyte adhesion to endothelial cells. In: Granger DN, Schmid-Schonbein GW (eds) Physiology and pathophysiology of leukocyte adhesion. Oxford University Press, New York, pp 148–168Google Scholar