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Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry

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The Low Molecular Weight Proteome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1023))

Abstract

Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is one of the most effective tools for localizing small molecules and compounds directly in thin tissue sections. MALDI IMS should be used when the distribution of molecular species is not known and to localize changes due to a disease process or a treatment. In recent years it has become increasingly clear that many pathological processes are not readily correlated to dramatic changes in protein levels. MALDI IMS can aid the localization of areas where the cellular concentration of proteins may be high enough to play an important biological role, but when the precise location is unknown. Here, we present a MALDI IMS protocol and data analysis of molecular imaging of multiple rat brain sections.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Bioscience, Drug safety and Toxicology, Uppsala University, Uppsala, Sweden

    Anna Ljungdahl & Malin Andersson

  2. Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden

    Jörg Hanrieder & Jonas Bergquist

Authors
  1. Anna Ljungdahl
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  2. Jörg Hanrieder
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  3. Jonas Bergquist
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  4. Malin Andersson
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Editor information

Editors and Affiliations

  1. , Department of Oncology-Pathology, Karolinska Institutet, N/A, Stockholm, 17176, Sweden

    Helena Bäckvall

  2. , Department of Oncology-Pathology, Karolinska Institutet, n/a, Stockholm, 171 76, Sweden

    Janne Lehtiö

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Ljungdahl, A., Hanrieder, J., Bergquist, J., Andersson, M. (2013). Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry. In: Bäckvall, H., Lehtiö, J. (eds) The Low Molecular Weight Proteome. Methods in Molecular Biology, vol 1023. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7209-4_7

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  • DOI: https://doi.org/10.1007/978-1-4614-7209-4_7

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-7167-7

  • Online ISBN: 978-1-4614-7209-4

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